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Development of a colloidal gold-based strip test for the detection of chlorothalonil residues in cucumber

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An immunochromatographic lateral flow strip test using the competitive format was developed for the determination of chlorothalonil (CTN) residues in cucumber. The limit of detection was less than 100 ng/mL by visual assessment and was found to be 91.78 ± 0.17 ng/mL for quantitative detection using a test strip reader. The recoveries of test samples were from 89.26% to 102.02% and the coefficient of variation (%) was less than 4.75%. Parallel analysis of cucumber samples with CTN showed comparable results from the strip test and high performance liquid chromatography. The strip test requires 5–8 min to complete and provides a very useful screening method for the quantitative and qualitative detection of CTN residues in cucumber.
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Keywords: chlorothalonil; colloidal gold; immunoassay; rapid detection; strip test

Document Type: Research Article

Affiliations: 1: Department of Veterinary Science, College of Life Science, Sichuan Agricultural University, Ya'an Sichuan, 625014, P.R. China 2: Department of Immunodetection, Key Laboratory for Animal Immunology of the Ministry of Agriculture, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, P.R. China

Publication date: September 3, 2015

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