Development of a colloidal gold-based strip test for the detection of chlorothalonil residues in cucumber
An immunochromatographic lateral flow strip test using the competitive format was developed for the determination of chlorothalonil (CTN) residues in cucumber. The limit of detection was less than 100 ng/mL by visual assessment and was found to be 91.78 ± 0.17 ng/mL for quantitative
detection using a test strip reader. The recoveries of test samples were from 89.26% to 102.02% and the coefficient of variation (%) was less than 4.75%. Parallel analysis of cucumber samples with CTN showed comparable results from the strip test and high performance liquid chromatography.
The strip test requires 5–8 min to complete and provides a very useful screening method for the quantitative and qualitative detection of CTN residues in cucumber.
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Document Type: Research Article
Department of Veterinary Science, College of Life Science, Sichuan Agricultural University, Ya'an Sichuan, 625014, P.R. China
Department of Immunodetection, Key Laboratory for Animal Immunology of the Ministry of Agriculture, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, P.R. China
September 3, 2015
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