@article {Zhao:2015:0954-0105:601,
title = "Soybean allergen glycinin induced the destruction of the mechanical barrier function in IPEC-J2",
journal = "Food and Agricultural Immunology",
parent_itemid = "infobike://tandf/cfai",
publishercode ="tandf",
year = "2015",
volume = "26",
number = "4",
publication date ="2015-07-04T00:00:00",
pages = "601-609",
itemtype = "ARTICLE",
issn = "0954-0105",
eissn = "1465-3443",
url = "https://www.ingentaconnect.com/content/tandf/cfai/2015/00000026/00000004/art00012",
doi = "doi:10.1080/09540105.2014.998638",
keyword = "glycinin, intestinal permeability, tight junction, food allergy, IPEC-J2",
author = "Zhao and Liu and Han and Zhang and Zhang and Qin",
abstract = "Humans and animals suffer a health threat to soybean allergy. The impaired mechanism of the intestinal barrier by food/feed allergen glycinin has not been clear. The effects of epithelial metabolic activity, permeability, integrity, and the tight junction (TJ) expression induced by
glycinin were conducted using porcine intestinal epithelial cells (IPEC-J2) model in this study. The results showed the decreased metabolic activity (R
2 = 0.964, p R
2 = 0.528, p =
0.002) linearly correlated with glycinin levels (04 mg/mL). The trans-epithelial electrical resistance values declined stimulated by 4 mg/mL glycinin treatment in a time-dependent manner (0, 6, 12, or 24 h, p = 0.018), and also descended after 12 or 24 h glycinin treatment in
dose-dependence (0, 0.5, 1, 2, or 4 mg/mL, p p < 0.05).",
}