@article {Sun:2015:0954-0105:577,
title = "IgE- and T-lymphocyte-Dependent Hypersensitivity Responses Induced in Mice by Exposure to Shrimp (Fenneropenaeus chinensis) Proteins",
journal = "Food and Agricultural Immunology",
parent_itemid = "infobike://tandf/cfai",
publishercode ="tandf",
year = "2015",
volume = "26",
number = "4",
publication date ="2015-07-04T00:00:00",
pages = "577-589",
itemtype = "ARTICLE",
issn = "0954-0105",
eissn = "1465-3443",
url = "https://www.ingentaconnect.com/content/tandf/cfai/2015/00000026/00000004/art00010",
doi = "doi:10.1080/09540105.2014.998635",
keyword = "shrimp (Fenneropenaeus chinensis), IgE-dependent hypersensitivity, allergen, mast cell degranulation, T lymphocyte",
author = "Sun and Fu, Ling-Lin and Wang",
abstract = "Shellfish allergy is a common cause of food-induced anaphylaxis in the human, and shrimp is one of the most frequently reported causes of allergic reactions. To identify the immunoglobulin E (IgE)-binding proteins of Chinese shrimp (Fenneropenaeus chinensis), the crude extracts
were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDSPAGE) and Western blotting. After exposure of shrimp extracts to mice, the levels of shrimp allergen-specific IgE were determined by indirect ELISA. Histological and immunohistochemical staining of intestinal
tract were further performed for immunological analysis. IgE-binding components with molecular masses of 40, 37, 28, 22, and 20 kDa at a high frequency are supposed to be major allergens of F. chinensis. In vivo experiments showed that shrimp extracts could lead to allergen-specific
IgE reactivity, and increase mast cell degranulation and numbers of T lymphocytes. IgE- and T-lymphocyte-dependent hypersensitivity plays important roles in allergic reactions triggered by F. chinensis allergens.",
}