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Immunological appraisal of wheat varieties in relation to chapatti-making characteristics

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The current research work was conducted to characterize wheat proteins through immunochemical techniques and to find out their relationship with wheat quality traits. The results revealed that wheat variety AARI-11 possessed higher protein content (11.96%), wet gluten (31.39%), dry gluten (9.66%), Pelshenke value (190.52 min), and SDS-Sedimentation value (28.27 ml) than other tested varieties. The chapattis prepared from the wheat variety AARI-11 got significantly higher sensory scores owing to its higher protein contents. The wheat variety AARI-11 also exhibited significantly the highest antibody response against all the assessed protein fractions. The results of the present study suggest that anti-glutenin and anti-high molecular weight glutenin subunits (HMW-GS) antibody response was found positively correlated to the quality characteristics of flours and chapattis. The present study suggests that the use of antibodies response against glutenin and HMW-GS offers good tool for predicting quality and suitability of wheat to chapatti-making quality.
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Keywords: ELISA; antibody response; chapatti; gluten; wheat proteins

Document Type: Research Article

Affiliations: 1: Department of Food Technology, PMAS-Arid Agriculture University, Rawalpindi, Pakistan 2: National Institiute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan

Publication date: July 4, 2015

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