
Immunological appraisal of wheat varieties in relation to chapatti-making characteristics
The current research work was conducted to characterize wheat proteins through immunochemical techniques and to find out their relationship with wheat quality traits. The results revealed that wheat variety AARI-11 possessed higher protein content (11.96%), wet gluten (31.39%), dry
gluten (9.66%), Pelshenke value (190.52 min), and SDS-Sedimentation value (28.27 ml) than other tested varieties. The chapattis prepared from the wheat variety AARI-11 got significantly higher sensory scores owing to its higher protein contents. The wheat variety AARI-11 also exhibited significantly
the highest antibody response against all the assessed protein fractions. The results of the present study suggest that anti-glutenin and anti-high molecular weight glutenin subunits (HMW-GS) antibody response was found positively correlated to the quality characteristics of flours and chapattis.
The present study suggests that the use of antibodies response against glutenin and HMW-GS offers good tool for predicting quality and suitability of wheat to chapatti-making quality.
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Keywords: ELISA; antibody response; chapatti; gluten; wheat proteins
Document Type: Research Article
Affiliations: 1: Department of Food Technology, PMAS-Arid Agriculture University, Rawalpindi, Pakistan 2: National Institiute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan
Publication date: July 4, 2015
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