Skip to main content
padlock icon - secure page this page is secure

Anti-melanogenic properties of honeybee (Apis mellifera L.) venom in α-MSH-stimulated B16F1 cells

Buy Article:

$63.00 + tax (Refund Policy)

Since ancient times, honeybee (Apis mellifera L.) venom (BV) has been applied to the skin as an anti-ageing remedy. To further access BV as an effective whitening agent for cosmetics and potential external treatment for topical use, we investigated its ability to inhibit tyrosinase activity and subsequent melanin biosynthesis. B16F1 melanoma cells were treated with 10 nM α-melanocyte stimulating hormone (α-MSH) and then with various doses of BV. BV inhibited direct tyrosinase activity and cellular tyrosinase activity, which decreased melanin synthesis in α-MSH-stimulated B16F1 melanoma cells. In addition, we used reverse transcription-polymerase chain reaction and Western blotting for melanogenesis-related genes such as tyrosinase-related protein (TRP)-1 and TRP-2 to examine the mechanisms underlying the inhibitory effects of BV on melanogensis. BV decreased the mRNA and protein expression of TRP-1 and TRP-2. These findings suggest that BV induced the downregulation of melanogenesis by inhibiting TRP-1 and TRP-2 activation.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: TRP-1; TRP-2; bee venom; melanin; tyrosinase; α-MSH

Document Type: Research Article

Affiliations: 1: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Suwon, South Korea 2: School of Biomedical Sciences, Charles Sturt University, Bathurst, Australia

Publication date: May 4, 2015

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more