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Development of a monoclonal antibody-based immunochromatographic strip for cephalexin

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An anti-cephalexin (CEX) monoclonal antibody was prepared by the immunogen of CEX-keyhole limpet hemocyanin connected by disuccinimidyl suberate, which was used for the establishment of a rapid immunochromatographic test strip. With this semi-quantitative detection method, a low limit of deduction of 10 ng mL−1 was obtained with the naked eyes and 1.3 ± 0.1 ng mL−1 by a strip reader in unprocessed milk within 5 min, which was much lower than the European Union Maximum Residue Limit of 100 µg kg−1 in milk. The recovery in the range of 87–120% was measured in another milk sample. In conclusion, this method with high sensitivity and satisfactory recovery shows significant potential for CEX residue detection in milk.
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Keywords: cephalexin; milk; monoclonal antibody; test strip

Document Type: Research Article

Affiliations: 1: State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi, China 2: Jiangsu Entry and Exit Inspection and Quarantine Bureau, Nanjing, China

Publication date: March 4, 2015

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