Skip to main content
padlock icon - secure page this page is secure

Plasmin-digest of β-lactoglobulin with antibacterial properties

Buy Article:

$63.00 + tax (Refund Policy)

The objective of the present study was to identify the antibacterial properties of plasmin digest of β-lactoglobulin (PDβ). After enzymatic treatment, PDβ was fractionated using reversed-phase high-performance liquid chromatography. The antibacterial activity of plasmin, β-lactoglobulin, PDβ, fractionated peptides and nisin as a control was tested against pathogenic (Escherichia coli and Staphylococcus aureus) and probiotic (Lactobacillus casei and Lactobacillus acidophilus) bacteria. Four antibacterial peptides, βL1, βL2, βL3 and βL5, PDβ and nisin as a control revealed antibacterial activity against pathogenic and probiotic bacteria tested. Finally, the identification of the antibacterial peptides was carried out using LC-Mass. Four antibacterial peptides showed molecular masses of 673.116, 819.320, 916.407 and 2410.515 Da, respectively. Two identified antibacterial peptides were βL1 and βL3, which corresponded to residues 78–83 and 83–91 of bovine β-lactoglobulin. The results showed that PDβ as a source of antibacterial peptides can be considered for controlling bacterial contamination.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: antibacterial; bovine; peptide; plasmin; β-lactoglobulin

Document Type: Research Article

Affiliations: 1: Department of Food Science and Technology, Faculty of Food Science and Technology, Science and Research Branch Tehran, Islamic Azad University, Tehran, Iran 2: Department of Biochemistry, Kharazmi University, Tehran, Iran 3: Department of Science, Faculty of Biology, Islamic Azad University Tehran Central Branch, Tehran, Iran 4: Department of Animal Science and Technology, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran

Publication date: March 4, 2015

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more