Skip to main content
padlock icon - secure page this page is secure

Immunochemical and molecular characteristics of monoclonal antibodies against organophosphorus pesticides and effect of hapten structures on immunoassay selectivity

Buy Article:

$63.00 + tax (Refund Policy)

To produce broad-selective monoclonal antibody (Mab) for organophosphorus (OP) pesticides, heterologous enzyme-linked immunosorbent assays (ELISAs) were used for hybridoma screening. Six Mabs with different cross-reactivity to OP pesticides were produced. Based on the broad-selective Mab 5F7, homologous and heterologous indirect competitive ELISAs were developed and the influence of hapten structure on assay selectivity was investigated. Moreover, the receptor–ligand interactions between 5F7 and OP pesticides were simulated to evaluate the broad-selectivity of the Mab. Results showed that, selectivity of the Mab was determined by its molecular properties, and the assay selectivity could be modified when the assay sensitivity was improved sufficiently by heterology. With the most suitable competitor, a broad-selective heterologous ELISA was developed. The IC50 values were estimated to be 7.06 ng/mL for parathion, 32.34 ng/mL for methyl parathion, 164.84 ng/mL for fenitrothion, 96.97 ng/mL for phoxim and 500.94 ng/mL for fenthion.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: enzyme-linked immunosorbent assay (ELISA); heterologous; molecular simulation; monoclonal antibody (Mab); organophosphorus pesticides

Document Type: Research Article

Affiliations: 1: Institute for the Control of Agrochemicals, Ministry of Agriculture, Beijing, China 2: Chinese Association of Agricultural Science Societies, Beijing, China 3: Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou, China

Publication date: January 2, 2015

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more