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Effect of instant controlled pressure drop (DIC) treatment on milk protein's immunoreactivity

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In this work, we tried to investigate the impact of treatment with instant controlled pressure drop technology (DIC) on the allergenicity of milk proteins. In DIC treatment, milk proteins are exposed to a high-pressure saturated steam (0.4 MPa, 144 °C) during 25 s and ended with an abrupt pressure drop towards a vacuum (5 kPa, 32 °C). Immunoglobulin E (IgE)-binding ability is determined with indirect enzyme linked immuno spectrophotometric analysis and western blot test. Results indicate significant changes in the IgE binding of treated proteins with DIC. Treated caseins showed increase in the IgE-binding ability; this indicates that DIC enhance the antigenicity of treated caseins. In the opposite, treated whey proteins (ß-lactoglobulin and α-lactalbumin) showed decrease in the IgE binding of these proteins. DIC treatment at 0.4 MPa during 25 s causes a greater reduction of the allergenicity of whey proteins than treatment with 0.6 MPa for 25 s.
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Keywords: DIC treatment; ELISA; caseins; electrophoresis; fluorescence; immunoreactivity; whey proteins

Document Type: Research Article

Affiliations: 1: Laboratoire de Nutrition et Technologie Alimentaire (L.N.T.A.), Institut de Nutrition et Technologies Agro-Alimentaires (I.N.A.T.A.A.), Université Constantine 1, Constantine, Algeria 2: INRA-Nantes, Unité des Biopolymères, Interactions et Assemblage (B.I.A.), équipe fonctions et interactions des protéines (F.I.P), Nantes, France 3: Laboratory of Engineering Science for Environment, LaSIE FRE-CNRS 3474, University of La Rochelle, La Rochelle, France 4: Laboratoire de nutrition et technologies alimentaires (L.N.T.A.), équipe Transformation et Elaboration des Produits Agro-alimentaires (T.E.P.A.), INATAA Université Constantine 1, Constantine, Algeria

Publication date: January 2, 2015

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