Three hapten derivatives of Sudan I were designed. Hapten-M and hapten-T shared a similar structure, differing only in spacer length. Hapten-X was obtained by reacting with the hydroxyl of Sudan I. Then polyclonal antibodies were produced and different combinations
of antibodies and coating antigens were designed. The results showed that it was not the spacer length of hapten but the hydroxyl played an indispensable role in inducing antibody production for Sudan I. More importantly, we verified the experimental results theoretically by calculating the
charge distribution of atoms. Next, monoclonal antibody was prepared and the optimal combination of monoclonal antibody and coating antigen was selected. The IC50 was 0.65 ng/mL for Sudan I, and good cross-reactivity of antibody to other azo compounds was obtained. Finally, we acquired
the recoveries of 82.2–116.0% and coefficients of variation of 4.3– 10.9% through the analysis of chilli sauce, tomato paste and chilli powder.
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Document Type: Research Article
State Key Laboratory of Pharmaceutical Biotechnology, Model Animal Research Center (MARC) of Nanjing University, Nanjing University, Nanjing, China
Key Lab of Mesoscopic Chemistry of MOE, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China
January 2, 2015
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