Skip to main content
padlock icon - secure page this page is secure

Development of a rapid and sensitive enzyme-linked immunosorbent assay to detect clonidine residues in swine urine samples

Buy Article:

$63.00 + tax (Refund Policy)

Clonidine (CLD) may be illegally used on animals because it can play the role of growth promoters. For the first time, a rapid direct competitive enzyme-linked immunosorbent assay (ELISA) was established based on polyclonal antibodies to detect CLD. The antibody showed high sensitivity, with the limit of detection (LOD) and IC50 being 0.01 and 0.056 µg/L, respectively. The specificity of the assay was evaluated by the measurement of cross-reactivity (CR) of the antibody with related compounds. Recovery rates ranged from 88% to 94%, and the coefficient of variation was below 11% for intra- and inter-assays. Highly consistent results were obtained for the urine samples by ELISA and UPLC-MS/MS method. It indicates good parallelism, accuracy and precision of the assay. The prototype kit could be advantageously used for the screening of large groups of urine samples, and has reliability even in routine application for the control of the illegal use of the drug.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: ELISA; clonidine; polyclonal antibody; residues; α-agonists

Document Type: Research Article

Affiliations: National Reference Laboratory of Veterinary Drugs Residues, China Institute of Veterinary Drug Control, Beijing, China

Publication date: January 2, 2015

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more