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Development of an ELISA for detection of Sudan I in food samples using monoclonal antibody

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A highly sensitive and specific indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) has been developed using a new monoclonal antibody for detecting the food colourant Sudan I. The half-maximum inhibition concentrations (IC50) and the limit of detection (calculated as IC20) of ELISA for Sudan I were 2 and 0.01 ng mL−1, respectively. The study showed little cross-activity with Sudan I structural analogues (below 0.01%). The average recoveries in intra- and interassays for Sudan I from fortified fresh tomato and chilli samples by ELISA were in ranges of 82–94% and 79–91%, respectively. The coefficients of variation of intra- and interassays were 6–8% and 6–10%, respectively. The IC50 was approximately 2.0 ng mL−1 after the Sudan I-Kit had been kept for 180 days.
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Keywords: ELISA; Sudan I; chilli; fresh tomato; monoclonal antibody

Document Type: Research Article

Affiliations: 1: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Science, Zhengzhou, Henan, PR China 2: College of Animal Science and Technology, Northwest Agriculture & Forest University, Yangling, Shanxi, PR China

Publication date: October 2, 2014

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