A highly sensitive and specific indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) has been developed using a new monoclonal antibody for detecting the food colourant Sudan I. The half-maximum inhibition concentrations (IC50) and the limit of detection (calculated
as IC20) of ELISA for Sudan I were 2 and 0.01 ng mL−1, respectively. The study showed little cross-activity with Sudan I structural analogues (below 0.01%). The average recoveries in intra- and interassays for Sudan I from fortified fresh tomato and chilli samples
by ELISA were in ranges of 82–94% and 79–91%, respectively. The coefficients of variation of intra- and interassays were 6–8% and 6–10%, respectively. The IC50 was approximately 2.0 ng mL−1 after the Sudan I-Kit had been kept for 180 days.
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Document Type: Research Article
Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Science, Zhengzhou, Henan, PR China
College of Animal Science and Technology, Northwest Agriculture & Forest University, Yangling, Shanxi, PR China
October 2, 2014
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