Skip to main content
padlock icon - secure page this page is secure

Preparation of anti-melamine antibody and development of an indirect chemiluminescent competitive ELISA for melamine detection in milk

Buy Article:

$63.00 + tax (Refund Policy)

An indirect chemiluminescent competitive ELISA (CL-ELISA) method was developed to detect residues of melamine in milk. A high-quality polyclonal antibody towards melamine cyanurate (MC) was prepared based on synthesis of a novel immunogen. The method is applicable over the range of 0.5–7.0 µg.mL−1 of MC, with an IC50 value of 1.7 µg.mL−1. The developed method was used in the detection of melamine residue in milk with the detection limit of 1 ng.mL−1. There was no cross-reactivity with commonly used veterinary drugs. The CL-ELISA method developed provides an alternative to chromatography spectrometry for regulatory analysis of melamine in milk and could be promisingly used to improve the sensitivity of the available ELISA test kits.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: antibody; chemiluminescent ELISA (CL-ELISA); drug residue; melamine

Document Type: Research Article

Affiliations: 1: The School of Chemistry and Chemical Engineering, Shandong University, Jinan, PR China 2: College of Pharmacy, Nankai University, Tianjin, PR China 3: Central Laboratory of Shandong Academy of Agricultural Sciences, Jinan, PR China

Publication date: October 2, 2014

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more