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Development of an enzyme-linked immunosorbent assay for octylphenol

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An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the quantitative analysis of octylphenol. A linear carboxylated analogue of 4-nonylphenol was synthesised and characterised as hapten. The hapten was coupled to the carrier protein to produce a specific antibody. An indirect competitive ELISA method was established based on the polyclonal antibody, which exhibited an IC50 value of 51 ng/mL for octylphenol. A monoclonal antibody was produced with an IC50 value of 76 ng/mL for octylphenol. The immunoassay used with the monoclonal antibodies was applied to analyse water samples from Tai Lake in China.
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Keywords: ELISA; antibody; monoclonal; octylphenol

Document Type: Research Article

Affiliations: State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi, China

Publication date: July 3, 2014

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