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Enzyme-linked immunosorbent assay–based two different polyclonal antibodies for the detection of cypermethrin with phenoxybenzene multiresidue

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Two kinds of new immunogens and coating antigens had been designed and synthesised by methods of lively ester (DCC) and carbodiimide (EDC), respectively. Preliminary immunosorbent assays indicated that the two target conjugates presented good inhibition ratio against the haptens of cypermethrin in vivo. The results showed that the best efficient titres of anti-cypermethrin rabbit serum in methods of DCC and EDC were 1:32000 and 1:64000, the IC50 of them were 18.907µg/L and 16.877µg/L against the haptens of cypermethrin, respectively. The data indicated that the anti-cypermethrin antibodies in method of EDC had higher sensitivity and higher selectivity than the anti-cypermethrin antibodies in method of DCC. Thus, we can synthesise antigens and anti-bodies with method of EDC. The antibodies of high quality can be effectively used in the field of the rapid detection of pyrethroid pesticide residues in the research of the reagent strips.
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Keywords: DCC; EDC; cypermethrin; enzyme-linked immunosorbent assay; haptens

Document Type: Research Article

Affiliations: Institute of Food Engineering, College of Life & Environment Science, Shanghai Normal University, Shanghai, PR China

Publication date: July 3, 2014

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