Skip to main content
padlock icon - secure page this page is secure

Anti-bensulfuron methyl monoclonal antibody production and BSM-detecting indirect competitive enzyme-linked immunoassay establishment

Buy Article:

$63.00 + tax (Refund Policy)

Bensulfuron methyl (BSM) is widely used for agricultural purposes and has raised health concerns, as well as ecological problems. Immunoassay would be one of the most advantaged measurements compared with traditional methods for BSM detection and measurement. In order to develop indirect competitive enzyme-linked immunoassay (icELISA), the anti-BSM monoclonal antibody (anti-BSM MAb) was produced. For the MAb production, BSM was conjugated to OVA and injected to mice with Freud's adjuvant for immunisation. Antiserum screening has revealed successful immunising. One stable hybridoma cell line (2H1) was obtained through cell fusion between spleen cells of immunised mouse and SP 2/0 cells. The MAb secreted by 2H1 cells was of high affinity and sensitivity, as well as specificity to BSM. Then the protocol of the icELISA and standard curve for BSM measurement was made and examined by controlled application. Significantly, the application has exhibited 96.530%–107.2% recovery of BSM. The produced MAb and established immunoassay may facilitate the measurement of BSM and herein help to ensure food safety and regulate environmental protection.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: anti-BSM monoclonal antibody (anti-BSM MAb); bensulfuron methyl (BSM); food safety; indirect competitive enzyme-linked immunoassay (icELISA)

Document Type: Research Article

Affiliations: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Huazhong Normal University, Wuhan, China

Publication date: July 3, 2014

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more