Skip to main content
padlock icon - secure page this page is secure

General immunoassay for pyrethroids based on a monoclonal antibody

Buy Article:

$63.00 + tax (Refund Policy)

For the development of class-specific antibody against pyrethroids, four haptens were designed and synthesised. A hybridoma 3E9 was successfully selected for production of antibody, which originated from mouse immunised with conjugates of hapten 1 [(RS)-cyano-3-phenoxybenzyl-2, 2, 3, 3-tetramethylcyclopropane-carboxylic acid] and keyhole limpet hemocyanin. A sensitive general immunoassay was developed with a monoclonal antibody from 3E9 cell line and coating antigen from conjugates of hapten 4 [N-(3-phenoxybenzoyl)-4-amino-L-phenylalanine] and bovine serum albumin. Under the optimised conditions, the antibody has good recognition to cypermethrin with 50% inhibition concentration (IC50) value of 1.7±0.76 ng mL−1. The cross-reaction to analogues was calculated as 12% for fenpropathrin, 4% for esfenvalerate. Besides, the antibody showed affinity to bifenthrin, deltamethrin and fenvalerate in different degrees with IC50 value ranging from 191.8 to 298.5 ng mL−1. The performance of this immunoassay was evaluated by fortified real water samples with three representative compounds. Recoveries were 76–118%, and the results showed that this immunoassay could be applied to monitor pyrethroid residues.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: immunoassay; monoclonal antibody; pyrethroid; water

Document Type: Research Article

Affiliations: 1: School of Food Science & Technology, State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi, China 2: Research Centre of Hunan Entry-Exit Inspection and Quarantine Bureau, Changsha, China

Publication date: July 3, 2014

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more