Skip to main content
padlock icon - secure page this page is secure

An in vitro study of Sanchi (Panax pseudoginseng) for its DNA protective effect

Buy Article:

$63.00 + tax (Refund Policy)

Panax pseudoginseng (Shachi) are well known for their antioxidant properties. The aim of this study was to explore Sanchi extracts, potential protection of human cellular DNA from oxidative challenge induced by hydrogen peroxide in vitro. Aqueous and alcoholic extracts of Shachi were incubated with human lymphocytes from 1×10−2% to 1×10−7% (w/v) for 30 mins. Pre-treated cells were then stressed with 50 µM hydrogen peroxide. Washed cells were subjected to the standard and the lysed cell comet assays. Results showed that water extracts of Sanchi at specific concentrations were able to diminish human DNA damage induced by hydrogen peroxide in both standard and lysed cell comet assays. No effect was observed in ethanolic extract. More pronounced protective effect in lysed cell comet assay model implied cell membrane limited the availability of active ingredients of Sanchi to nucleus.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: DNA; acellular comet assay; hydrogen peroxide; lysed cell; oxidative challenge

Document Type: Research Article

Affiliations: 1: Department of Applied Science, Hong Kong Institute of Vocational Education, Sha Tin, Hong Kong 2: Department of Pathology, Kwong Wah Hospital, Kowloon, Hong Kong 3: Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Kowloon, Hong Kong 4: School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia

Publication date: July 3, 2014

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more