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An in vitro study of Sanchi (Panax pseudoginseng) for its DNA protective effect

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Panax pseudoginseng (Shachi) are well known for their antioxidant properties. The aim of this study was to explore Sanchi extracts, potential protection of human cellular DNA from oxidative challenge induced by hydrogen peroxide in vitro. Aqueous and alcoholic extracts of Shachi were incubated with human lymphocytes from 1×10−2% to 1×10−7% (w/v) for 30 mins. Pre-treated cells were then stressed with 50 µM hydrogen peroxide. Washed cells were subjected to the standard and the lysed cell comet assays. Results showed that water extracts of Sanchi at specific concentrations were able to diminish human DNA damage induced by hydrogen peroxide in both standard and lysed cell comet assays. No effect was observed in ethanolic extract. More pronounced protective effect in lysed cell comet assay model implied cell membrane limited the availability of active ingredients of Sanchi to nucleus.
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Keywords: DNA; acellular comet assay; hydrogen peroxide; lysed cell; oxidative challenge

Document Type: Research Article

Affiliations: 1: Department of Applied Science, Hong Kong Institute of Vocational Education, Sha Tin, Hong Kong 2: Department of Pathology, Kwong Wah Hospital, Kowloon, Hong Kong 3: Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Kowloon, Hong Kong 4: School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia

Publication date: July 3, 2014

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