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Identification of a second major antigenic epitope in the α-subunit of soy β-conglycinin

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Soybean meal in the post-weaning diet of pigs results in a growth lag correlated with Immunoglobulin G (IgG) production against soybean proteins, including the seed storage protein β-conglycinin. We screened sera from 30 pigs for IgG directed against β-conglycinin. Analysis of the same sera on protein blots identified sera that had IgG against the α-subunit of β-conglycinin. A tiled peptide array (16 amino acids with an 11 amino acid overlap) of the α-subunit of β-conglycinin was screened to identify the epitopes of the α-subunit that bind IgG. One major IgG binding epitope previously identified was confirmed. A second major IgG binding epitope was identified that bound IgG from half of the animals tested. Minor epitopes that bound IgG from one or two other sera were also identified. Binding of IgG to β-conglycinin in an enzyme-linked immunosorbent assay (ELISA) was inhibited by a synthetic peptide representing one of the minor epitopes.
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Keywords: IgG; allergen; epitope; pig; seed storage protein; soybean; swine

Document Type: Research Article

Affiliations: 1: USDA/ARS, Raleigh, NC, USA 2: Department of Animal Science, North Carolina State University, Raleigh, NC, USA

Publication date: July 3, 2014

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