This study investigates the possible mechanism of the protective effects of blueberry anthocyanins on human embryonic liver L-02 cells. Results of the WST-8 method showed that different concentrations of blueberry anthocyanins protected the human embryonic liver L-02 cells against CCl4-induced
injury in a dose-dependent manner with IC50 at 6.2×g/L. Cell clone formation inhibition assay demonstrated that cell clones increased with increased concentration of anthocyanins. Propidium iodide (PI) staining analysis showed that anthocyanins can decrease cell cycle in the
G1 phase. DNA ploidy analysis showed a weakened percentage of hypodiploid cells in a dose-dependent manner. Moreover, Annexin V-FITC/PI staining analysis showed a dose-dependent effect of anthocyanins on late apoptotic and necrotic cells. Western blot assay showed gradually decreased caspase-3
protein expression levels with increased anthocyanin concentration. In summary, these findings provided pharmacological evidence supporting the clinical application and protective effect of blueberry anthocyanins against acute liver injury.
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acute liver injury;
human embryonic liver cell (L-02)
Document Type: Research Article
Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing, China
Department of Food Science and Engineering, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
April 3, 2014
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