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Protective effect of blueberry anthocyanins in a CCl4-induced injury model in human embryonic liver cells

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This study investigates the possible mechanism of the protective effects of blueberry anthocyanins on human embryonic liver L-02 cells. Results of the WST-8 method showed that different concentrations of blueberry anthocyanins protected the human embryonic liver L-02 cells against CCl4-induced injury in a dose-dependent manner with IC50 at 6.2√óg/L. Cell clone formation inhibition assay demonstrated that cell clones increased with increased concentration of anthocyanins. Propidium iodide (PI) staining analysis showed that anthocyanins can decrease cell cycle in the G1 phase. DNA ploidy analysis showed a weakened percentage of hypodiploid cells in a dose-dependent manner. Moreover, Annexin V-FITC/PI staining analysis showed a dose-dependent effect of anthocyanins on late apoptotic and necrotic cells. Western blot assay showed gradually decreased caspase-3 protein expression levels with increased anthocyanin concentration. In summary, these findings provided pharmacological evidence supporting the clinical application and protective effect of blueberry anthocyanins against acute liver injury.
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Keywords: CCl4; acute liver injury; apoptosis; blueberry anthocyanins; human embryonic liver cell (L-02)

Document Type: Research Article

Affiliations: 1: Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing, China 2: Department of Food Science and Engineering, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China

Publication date: April 3, 2014

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