@article {Zhang:2014:0954-0105:256,
title = "Preparation and validation of monoclonal antibody-based indirect competitive ELISA for detecting testosterone levels",
journal = "Food and Agricultural Immunology",
parent_itemid = "infobike://tandf/cfai",
publishercode ="tandf",
year = "2014",
volume = "25",
number = "2",
publication date ="2014-04-03T00:00:00",
pages = "256-266",
itemtype = "ARTICLE",
issn = "0954-0105",
eissn = "1465-3443",
url = "https://www.ingentaconnect.com/content/tandf/cfai/2014/00000025/00000002/art00010",
doi = "doi:10.1080/09540105.2013.775221",
keyword = "bovine, testosterone, indirect competitive ELISA, monoclonal antibody",
author = "Zhang and Jiang and Wang and Chen and Wang and Ding and Li and Li",
abstract = "This paper presents the generation of monoclonal antibodies (mAbs) against testosterone (TES) through cell fusion technology, and the development of a mAb-based indirect competitive enzyme-linked immunosorbent assay (icELISA) method to detect TES residue in bovine edible tissues. Under
optimal conditions, this assay exhibited a working range of 0.0419 ng/mL, with half-maximum inhibition (IC50) and limit of detection values of 0.27 and 0.02 ng/mL, respectively. After three sample pretreatment procedures were checked, a simple dilution method was adopted
for further use. The stabilisation studies demonstrated that the icELISA kits can be stored for at least 180 to 240 days, at 4\textdegreeC and 20\textdegreeC, respectively. When applied to bovine samples, the data from icELISA and gas chromatography coupled to mass spectrometry showed excellent
correlation (r2=0.9923 in muscle, 0.9884 in liver and 0.9957 in kidney). Therefore, this assay has the potential to be incorporated into a quantitative monitoring programme for the rapid screening of TES residue in foods.",
}