Skip to main content
padlock icon - secure page this page is secure

Immunosorbent analysis of ricin contamination in milk using colorimetric, chemiluminescent and electrochemiluminescent detection

Buy Article:

$63.00 + tax (Refund Policy)

Analytical methodology to detect active ricin in food matrices is important because of the potential use of foodborne ricin as a terrorist weapon. Monoclonal antibodies (mAbs) that bind ricin were used as both capture and detection ligands in sandwich enzyme-linked immunosorbent assay (ELISA) and electrochemiluminescent (ECL) immunosorbent assays. ELISA employed two types of substrate for colorimetric or chemiluminescent detection. Although both fat content and protein content of samples influenced the recovery of ricin, the lower limit of detection (LOD) in ELISA and ECL systems permitted detection of 0.1 ng/mL for milk samples containing 0–4% fat. The assay systems detect pure ricin- or crude ricin-containing castor extract, but do not significantly respond to isolated ricin chains, heat-denatured ricin or the related agglutinin, Ricinus communis agglutinin 1 (RCA-1). Using the standard 96-well-plate formats, the assays detect less than 0.01% of an adult human lethal dose in a typical serving of milk.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: Ricinus communis agglutinin; castor; monoclonal antibody; ricin

Document Type: Research Article

Affiliations: Foodborne Contaminants Research Unit, Western Regional Research Center, USDA Agricultural Research Service, Albany, CA, USA

Publication date: April 3, 2014

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more