@article {Wang:2014:0954-0105:149,
title = "Improved preparation of a chitosan-based immunoaffinity column using antibody against methandrostenolone as ligand",
journal = "Food and Agricultural Immunology",
parent_itemid = "infobike://tandf/cfai",
publishercode ="tandf",
year = "2014",
volume = "25",
number = "2",
publication date ="2014-04-03T00:00:00",
pages = "149-159",
itemtype = "ARTICLE",
issn = "0954-0105",
eissn = "1465-3443",
url = "https://www.ingentaconnect.com/content/tandf/cfai/2014/00000025/00000002/art00001",
doi = "doi:10.1080/09540105.2012.753514",
keyword = "methandrostenolone, high-performance liquid chromatography, chitosan, immunoaffinity chromatography, enzyme-linked immunosorbent assay",
author = "Wang and Wang and Xu and Wu and Dong",
abstract = "Chitosan is obtained by N-deacetylation of chitin, which is the second most abundant natural biopolymer. In this study, an improved preparation and characterisation of a chitosan-based immunoaffinity chromatography (IAC) column were performed. The immunoaffinity adsorbent was prepared
by covalently coupling monoclonal antibody (mAb) against methandrostenolone (MA) to glutaraldehyde cross-linked chitosan (chitosan CL). Scanning electron micrograph of chitosan CL indicated that the shape of the particles was spherical with a diameter range from about 300 to 500 \textmum.
Infrared spectral analysis suggested that the immunoaffinity adsorbent was successfully prepared by coupling antibody with chitosan CL. For chromatographic extraction, 90% methanol was selected as eluant. Under optimum conditions, the maximum binding capacity of the IAC column was 3900 ng
MA/mL adsorbent. The average recovery of 50, 250 and 500 ng of MA standards from IAC columns was 96.9% with a relative standard deviation among columns of 1.48%. After eight uses, the extraction recovery of the IAC column remained 82.1%. To further verify the effect of matrices on the extraction
efficiency, the IAC columns were challenged with MA-fortified animal tissue and feed samples. Recoveries were 84.987.1%, demonstrating the effectiveness of these IAC columns for sample clean-up in MA residue determination.",
}