@article {Peng:2014:0954-0105:1,
title = "Development of a monoclonal antibody-based sandwich ELISA for the detection of ovalbumin in foods",
journal = "Food and Agricultural Immunology",
parent_itemid = "infobike://tandf/cfai",
publishercode ="tandf",
year = "2014",
volume = "25",
number = "1",
publication date ="2014-01-02T00:00:00",
pages = "1-8",
itemtype = "ARTICLE",
issn = "0954-0105",
eissn = "1465-3443",
url = "https://www.ingentaconnect.com/content/tandf/cfai/2014/00000025/00000001/art00001",
doi = "doi:10.1080/09540105.2012.716398",
keyword = "food allergen, monoclonal antibody, ovalbumin, sandwich ELISA",
author = "Peng and Meng and Deng and Zhu and Kuang and Xu",
abstract = "Hen egg is one of the most frequent causes of food allergy in infants and adults. Ovalbumin (OVA) has been identified as a major egg allergen. In order to detect OVA in foods, a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAbs)
was established. The 2 mAbs were selected out of 17 murine hybridomas secreting OVA-specific antibody. Using mAb17 as the capture antibody and mAb15 as the detection antibody, the detection limit of the ELISA method was 0.51 ng/mL, and the linear dynamic range was between 1.95 and 500 ng/mL.
The recovery ranged from 85.6 to 115.2%, whereas the intra- and inter-assay coefficients of variation were less than 8.6 and 13.9%, respectively. Sample analysis verified that the produced anti-OVA mAb and the developed ELISA may provide a valuable tool for the sensitive determination of OVA
in processed foods and for future studies on the mechanism of how OVA functions in anaphylaxis.",
}