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Assessment of cross-reactivity in patients allergic to birch pollen by immunoblotting

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Cross-reactivity related to birch pollynosis and ingestion of certain food poses a severe clinical and diagnostic problem. Sera were taken from 21 adult patients allergic to birch pollen with symptoms after ingestion of apples, carrots and celeries, and seven control subjects allergic to birch pollen only. Concentrations of allergen-specific IgE against allergens of birch pollen, apple, carrot and celery were measured with enzyme immunoassay, fluorimetric enzyme-linked immunoassay and immunoblotting. Immunoblotting technique may serve as a valuable diagnostic tool for birch pollen associated cross-reactivity.
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Keywords: birch pollen; cross reactivity; food allergy; immunoblotting; major allergens

Document Type: Research Article

Affiliations: 1: NCU in Bydgoszcz, Department of Allergology, Clinical Immunology and Internal Diseases, ul. Ujejskiego 75, Bydgoszcz, 85-168, Poland 2: Department of Biochemistry, Polish Academy of Science, ul. Ludwika Pasteura 3, Warszawa, 02-093, Poland

Publication date: December 1, 2013

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