The gastrointestinal distribution of glycinin in pigs at different growth stages was investigated in this study. Fifteen healthy General No. 1 barrows weaned on the 28th day were randomly assigned to three groups with five replicates: five weanling, five growing and five finishing.
All pigs received diets with non-soya bean ingredients in non-experimental periods, while the pigs received diets containing 4% purified glycinin in experimental periods. Binding of anti-glycinin antibody was detected using a labelled streptavidin–peroxidase complex method to determine
the gastrointestinal distribution of glycinin. The results indicated that there was a significant difference on the gastrointestinal mucosal distribution of glycinin between pigs of different ages. Glycinin in growers was higher than piglets and lower than finishers (P<0.001). The
highest content of glycinin was in the distal jejunum and ileum for piglets, and in distal jejunum for growers and finishers (P<0.05).
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Affiliations:1: Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, P.R.China 2: College of Animal Science and Technology, Jilin Agricultural University, Changchun, P.R.China