Using the direct competitive chemiluminescent enzyme immunoassay (CLEIA), a rapid, highly selective and sensitive method for carbofuran determination was developed. Several physicochemical parameters such as chemiluminescent sensitiser, assay buffer, blocking substance and working concentration
were optimised. Under the optimum conditions, the CLEIA method for carbofuran determination was generated successfully. The detection sensitivity of the IC50 value was 4.2 ng/mL at a practical working concentration ranging from 0.4 to 50 ng/mL, and the limit of detection (LOD) for
carbofuran was 0.5 ng/mL. Recoveries of carbofuran spiked into vegetable, fruit and environmental samples were determined by CLEIA after sample matrix effect testing. Finally, the confirmation test between CLEIA and LC-MS/MS was finished. The results showed that CLEIA method had higher sensitivity
than enzyme-linked immunosorbent assay method, and the LOD of a variety of samples reached the carbofuran's maximum residue limit value laid down by Codex Alimentarius Commission.
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Affiliations:1: Key Laboratory for Agro-Products Quality and Safety of Chinese Ministry of Agriculture, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing,
100081, China 2: Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou, 310029, China 3: Zhejiang Gongshang University, Hangzhou, 310035, China