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A sensitive chemiluminescent enzyme immunoassay for carbofuran residue in vegetable, fruit and environmental samples

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Using the direct competitive chemiluminescent enzyme immunoassay (CLEIA), a rapid, highly selective and sensitive method for carbofuran determination was developed. Several physicochemical parameters such as chemiluminescent sensitiser, assay buffer, blocking substance and working concentration were optimised. Under the optimum conditions, the CLEIA method for carbofuran determination was generated successfully. The detection sensitivity of the IC50 value was 4.2 ng/mL at a practical working concentration ranging from 0.4 to 50 ng/mL, and the limit of detection (LOD) for carbofuran was 0.5 ng/mL. Recoveries of carbofuran spiked into vegetable, fruit and environmental samples were determined by CLEIA after sample matrix effect testing. Finally, the confirmation test between CLEIA and LC-MS/MS was finished. The results showed that CLEIA method had higher sensitivity than enzyme-linked immunosorbent assay method, and the LOD of a variety of samples reached the carbofuran's maximum residue limit value laid down by Codex Alimentarius Commission.
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Keywords: assay optimisation; carbofuran; chemiluminescent enzyme immunoassay (CLEIA); ultra performance liquid chromatography (UPLC)-MS/MS

Document Type: Research Article

Affiliations: 1: Key Laboratory for Agro-Products Quality and Safety of Chinese Ministry of Agriculture, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China 2: Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou, 310029, China 3: Zhejiang Gongshang University, Hangzhou, 310035, China

Publication date: September 1, 2013

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