A survey of fungal contamination and presence of aflatoxins (AFs) and fumonisins (FBs) in 30 feed samples collected from 10 tilapia farms during three seasons in Nayarit State, located in north-western Mexico, was carried out using ELISA as screening and High-Performance Liquid Chromatography
(HPLC) as confirmatory method. Mycobiota included Aspergillus flavus and Fusarium spp. AFs were detected in 63.3% of samples using ELISA, but confirmation by HPLC revealed that all samples were under the detection limit. Regarding to FBs, positive samples were detected using
both methods, with 19 positive samples (60% of total) by ELISA and 14 positive samples (46.6% of total) by HPLC and levels ranging from 0.148 to 2.587 mg/kg. Correlation was observed between both methods (r = 0.516, p = 0.004) for FBs results. No sample exceeded the European
maximum levels for any of the mycotoxins. Water activity of samples ranged from 0.345 to 0.655, suggesting that mycotoxin occurrence is probably related to raw material contamination.
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Affiliations:1: Secretaría de Investigación y Posgrado, Cd de la Cultura Amado Nervo, Universidad Autónoma de Nayarit, Tepic, Nayarit C.P., 63190, México 2: Applied Mycology Unit, Department of Food Technology, XaRTA-UTPV-CRA, University of Lleida, Av. Rovira Roure 191, 25198, Lleida, Spain