Skip to main content
padlock icon - secure page this page is secure

Development of a broad-selective immunoassay for multi-residue determination of type II pyrethroids in West Lake water

Buy Article:

$63.00 + tax (Refund Policy)

With the purpose of finding an immunoassay of similar sensitivity for type II pyrethroids such as fenpropathrin, cypermethrin, deltamethrin, esfenvalerate, cyhalothrin, fluvalinate and flucythrinate, we prepared three kinds of polyclonal antibodies (Pabs), and screened against nine coating antigens (CAgs). The final selected antibody–antigen combination based on heterologous assay was further optimised and tested for tolerance to methanol concentration, ionic strength and pH changes. Sensitivity of optimal immunoassay was found to be quite similar among cypermethrin, deltamethrin and esfenvalerate, as IC50 values were 23.2, 24.5 and 25.1 µg/L for the three pyrethroids, respectively. No cross-reactivity was measured to type I pyrethroids or other relative metabolites. This immunoassay was used to detect different combinations of cypermethrin, deltamethrin and esfenvalerate spiked in West Lake water, and recoveries were between 95.6% and 103.6%, which indicated that the chosen ELISA was feasible for multi-residue analysis.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: West Lake water; broad-selective immunoassay; multi-residue; type II pyrethroids

Document Type: Research Article

Affiliations: Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou,310029, PR China

Publication date: March 1, 2013

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more