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Evaluation and characterization of the immunomodulatory activity of the protein extract from Citrullus colocynthis L.

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Citrullus colocynthis L. is a plant widely used in Moroccan traditional medicine as a strong laxative and has many others uses. The aim of the present work is to evaluate the immunomodulatory activity of the protein extract (PE) from seeds of C. colocynthis. The results of this study showed that the PE has an immunosuppressive activity in vitro. The activity was assessed through the evaluation of the proliferation of splenocytes induced by the mitogen concanavalin-A factor. The PE showed no agglutination activity against rabbit erythrocytes, and was separated into two fractions F1 and F2 with molecular weight of 160 and 79 kDa, respectively. Fraction F1 showed the highest immunosuppressive activity (44.62%) in dose-dependent manner (IC50 60 µg/mL) and is characterized into three sub-units of 53.65 kDa. The sub-units of F1 were determined by the polyacrylamide gel electrophoresis. The fraction F1 of the PE could be a promising immunosuppressing agent extracellular.
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Keywords: Citrullus colocynthis; concanavalin-A; gel electrophoresis; immunomodulatory; immunosuppressive; splenocytes

Document Type: Research Article

Affiliations: 1: Unit of Immunology and Pharmacology, Faculty of Science & Technology,Laboratory of Bioactive Molecules, Fez, Morocco 2: National Institute of Medicinal and Aromatic Plants, University Sidi Mohamed Ben Abdellâh, Taounate province, Fez, Morocco 3: Deptartment of Natural Products and Alternative Medicine, Faculty of Pharmacy,King Abdulaziz University, Saudi Arabia

Publication date: March 1, 2013

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