Skip to main content
padlock icon - secure page this page is secure

Electrochemiluminescence immunosorbent assay of ricin in ground beef: biotinylated capture antibodies and matrix effects

Buy Article:

$63.00 + tax (Refund Policy)

Ricin is a highly toxic protein present in the seeds of castor (Ricinus communis), grown principally as a source of high quality industrial lubricant. Because of the past use of ricin for intentional poisoning, there is a need for analytical methodology to detect ricin in food matrices. Ground beef and other fatty, solid matrices present challenges for extraction and detection of protein constituents. This study focused on the use of streptavidin-coated assay plates, with biotinylated monoclonal antibodies (mAbs) immobilised as capture reagents. It explored matrix effects on immunosorbent analyses of ricin in enzyme-linked and electrochemiluminescent detection systems. A variety of mAb pairs enabled assays with predetermined specificity for ricin vs. the related protein, Ricinus communis agglutinin-1 (RCA-1). Extraction of samples at low dilution (1:5) and inclusion of 100 mM galactose in the extraction medium produced excellent quantification of ricin in the 1–20 ng/g range in ground beef.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: Ricinus communis agglutinin; biothreat; castor; electrochemiluminescence; ground beef; monoclonal antibody; ricin

Document Type: Research Article

Affiliations: Foodborne Contaminants Research Unit, Western Regional Research Center,USDA Agricultural Research Service, 800 Buchanan StreetAlbany,CA,94710, USA

Publication date: December 1, 2012

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more