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Inter-laboratory comparison of Cry1Ab toxin quantification in MON 810 maize by enzyme-immunoassay

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A laboratory ring trial was performed in four laboratories for determination of Cry1Ab toxin in leaf material of MON 810 maize using a standardised enzyme-linked immunoassay protocol. Statistical analysis was carried out by the ISO 5725-2 guidelines, sample standard deviation and standard error, within-laboratory and inter-laboratory SD and SE were calculated. Measured inter-laboratory average values were 12.5±4.0, 15.3±4.6 and 72.6±17.8 µg/g for three lyophilised samples, and 27.8±4.3 µg/g for a frozen sample, yet, Cry1Ab concentrations ranged 66.5–160.1% of the corresponding IA. Determined concentrations by in-house protocols were statistically not different in one laboratory and different in two laboratories from the corresponding values by the joint protocol. Results emphasise the importance of a standardised protocol among laboratories for comparable quantitative Cry1Ab toxin determination. However, even when using a standardised protocol, significant differences still occur among toxin concentrations detected in different laboratories, although with a smaller range of variation.
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Keywords: Bacillus thuringiensis; Cry1Ab toxin; ELISA; GM plants; MON 810

Document Type: Research Article

Affiliations: 1: Department of Ecotoxicology and Environmental Analysis,Plant Protection Institute of the Hungarian Academy of Sciences, POB 102H-1525,Budapest, Hungary 2: Ecostrat GmbH, Friedensallee 2115834,Rangsdorf, Germany 3: Genøk Centre for Biosafety, POB 64189294,Tromsø, Norway

Publication date: June 1, 2012

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