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Development of a sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of cadmium ions in water, soil and rape samples

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Cadmium is classified as a probable human carcinogen. A monoclonal antibody (mAb) against Cd2+-ethylene-diamine-N,N,N′,N′-tetraacetic acid (EDTA) complex was generated by immunising mice with the conjugate of Cd2+-EDTA-aminobenzyl-ovalbumin. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed and applied for the analysis of Cd2+ in water, soil and rape samples. The half-maximum inhibition concentration and detection range were 2.59 ng/ml and 0.20–40 ng/ml, respectively. The assay cross-reactivities with non-target metal-EDTA complexes were all below 1%, except Mn2+ (2.9%) and Hg2+ (1.6%). The average recoveries of Cd2+ in tap water, Jingmi Canal, Xiaoqing River (at concentrations of 2–20 ng/ml), soil (0.25–10 ug/g) and rape (0.1–1.2 ug/g) samples were 103.0%, 101.0%, 103.0%, 82.4% and 93.7%, respectively. Concentrations of Cd2+ measured by this immunoassay agreed well with those determined by graphite furnace atomic absorption spectroscopy (GFAAS) (k=1.1225, r 2=0.9873).The established icELISA was a quick, reliable and sensitive method to detect Cd2+ in environmental and vegetable samples.
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Keywords: ELISA; cadmium; graphite furnace atomic absorption spectroscopy; immunoassay; monoclonal antibody

Document Type: Research Article

Affiliations: 1: College of Agronomy and Biotechnology,China Agricultural University, 100193,Beijing, China 2: Department of Molecular Biosciences and Bioengineering,University of Hawaii, Honolulu,HI,96822, USA 3: Institute of Natural Medicine and Chinese Medicine Resources,Beijing Normal University, 100875,Beijing, China

Publication date: March 1, 2012

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