Allergenic changes in β-lactoglobulin induced by microwave irradiation under different pH conditions
The allergenicity of β-lactoglobulin (BLG) was assayed by Ussing chamber after microwave irradiation of whey proteins at different pH values, in a murine model of BLG allergy. BALB/c mice were sensitised intraperitoneally with BLG. Serum levels of BLG-specific IgG, IgG1, IgG2a and IgE were analysed by Enzyme-Linked Immunosorbent Assay (ELISA). Local anaphylactic responses and residual allergenicity of various treated whey proteins were performed in vitro in Ussing chamber. BLG immunisation was associated with strong IgG, IgG1, IgG2a and IgE production, a significant increase in short current circuit (Isc) and high conductance (G) response. The allergenic potential of BLG was markedly reduced after microwave irradiation at 300 or 700 W of whey proteins at pH values 6.8 or 4.6 (Isc and G remained unchanged after intestine challenge with treated whey proteins). The application of microwave irradiation of whey proteins at the natural milk pH (pH 6.8) or pH 4.6 induces a significant decrease in the BLG allergenicity.
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Document Type: Research Article
Affiliations: Laboratory of Physiology of the Nutrition and Food Safety, Department of Biology, Faculty of Science,University of Oran, Es-Sénia, Oran-31000, Algeria
Publication date: December 1, 2011