This study evaluated the effects of dexamethasone and insulin-like growth factor-1 on mitogen-induced lymphocyte immune-associated activities including metabolic reduction of the tetrazolium salt and immunoglobulin (Ig) production. Peripheral blood lymphocytes were isolated from blood
samples obtained from three 6-week-old male pigs and plated in triplicate for treatment and dose (n=9 wells/treatment/dose). Cells were stimulated with specific doses and combinations of concanavalin A (ConA), pokeweed mitogen (PWM), DEX and/or IGF-1. Both Con A and PWM induced dose-dependent
increases (P<0.05) in lymphocyte metabolism and IgM production. Lymphocyte metabolism and IgM production induced by submaximal concentrations of ConA and PWM were dose-dependently suppressed (P<0.05) by DEX. Treatment with IGF-1 attenuated (P<0.5) the suppressive
effects of DEX on ConA- but not PWM-induced lymphocyte metabolism. The addition of IGF-1 reduced (P<0.05) DEX suppression of ConA- and PWM-induced IgM production. These results demonstrate that IGF-1 can differentially reduce the suppressive effects of DEX on lymphocytes dependent
on mitogen type.
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Document Type: Research Article
Department of Animal and Wildlife Sciences, College of Agriculture and Human Sciences,Texas A&M University-Kingsville, TX78363, USA
Texas AgriLife Research,Texas A&M University System, College StationTX77843, USA
USDA-ARS Livestock Issues Research Unit, LubbockTX79403, USA
December 1, 2011
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