Skip to main content
padlock icon - secure page this page is secure

Development and validation of an immunochromatographic test strip for rapid detection of doxycycline residues in swine muscle and liver

Buy Article:

$63.00 + tax (Refund Policy)

A one-step lateral flow immunochromatographic technique using colloidal gold-labelled monoclonal antibody (Mab) was developed for the rapid detection of doxycycline (DOX) residues in swine tissues. For this purpose, a Mab against DOX named as 2.3/3A6 with low cross-reactivity (CR) to other tetracyclines (TCs) was produced. The sensitivity of the test strip was as low as 7 ng/mL for DOX and the half maximal inhibitory concentration (IC50) was calculated to be 22±2 ng/mL by relative optical density. The test can be completed within 10 min and the detection limit was 20 ng/mL in unaided visual assessment. Recoveries in samples spiked with DOX at concentrations of 20, 40 and 80 ng/g, were demonstrated to be from 81% to 95% for muscle samples and from 81% to 92% for liver samples. The intra-assay and inter-assay coefficients of variation (CVs) ranged from 3% to 6% and from 4% to 8%, respectively. Comparing with high performance liquid chromatography (HPLC) method in sensitivity and accuracy for the detection of DOX in swine tissues, the test strip showed good agreement. Therefore, the test strip is suitable for rapid and reliable determination of DOX residues in swine tissues semi-quantitatively or qualitatively.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: colloidal gold; doxycycline; immunochromatography; monoclonal antibody (Mab); test strip

Document Type: Research Article

Affiliations: 1: College of Life Science,Chongqing Normal University, Chongqing401331, P.R. China 2: National Reference Laboratory of Veterinary Drug Residues,Huazhong Agricultural University, Wuhan430070, P.R. China 3: State Key Laboratory of Agricultural Microbiology,College of Veterinary Medicine, Huazhong Agricultural University, Wuhan430070, P.R. China

Publication date: September 1, 2011

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more