A rapid, sensitive immunochromatographic (IC) assay was developed to detect ricin toxin in assay buffer and food samples. The assay was based on the sandwich format using a monoclonal antibody (mAb) and a polyclonal antibody (pAb) of two distinct specificities. The ricin-containing
sample was added to the membrane and allowed to react with pAb-coated particles. The mixture was then passed along the porous membrane by capillary action past the mAb in the detection zone, which would bind the particles that had ricin bound to their surfaces, giving a red colour within the
detection zone with an intensity proportional to the ricin concentration. In the absence of ricin, no immunogold was bound to the solid-phase antibody. With this method, 30 ng/ml of ricin toxin was detected in less than 10 min, and the assay also showed no cross-reactivity with abrin toxin.
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immunochromatographic test strip;
Document Type: Research Article
Tianjin Key Lab for Biomarkers Occupational and Environmental Hazard, Medical College of Chinese People's Armed Police Force, China
The State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing, People's Republic of China
June 1, 2011
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