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Generation of antibody and development of an enzyme-linked immunosorbant assay for the feed additive roxarsone

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Roxarsone is used in the poultry and swine industries as a feed additive to treat coccidiosis and other intestinal disorders as well as to improve feed efficiencies and weight gain. 3-Amino-4-hydroxyphenylarsonic acid (AHPA) was conjugated with keyhole limpet hemocyanin in the presence of bis-sulfosuccinimidyl-suberate for the immunogen in rabbits. The antibody has minor cross-reactivity towards 2-aminophenylarsonic acid (7.3%, n=3), arsanilic acid (5.3%, n=3), AHPA (4%, n=3), and phenylarsonic acid (3%, n=3). The mean IC50s for PBST, muscle 1:10, and muscle 1:20 were 11.7±1.50, 13.1±2.53 and 12.0±2.45 ng/mL (n=6). Using a standard curve prepared in chicken muscle extract and diluted with PBST at 1:20, muscle samples spiked with roxarsone at 2.5, 5, 10 and 25 ng/mL had recoveries of 117%, 98%, 80% and 65% having coefficients of variation of 36, 9.2, 10.2 and 11.5 respectively (n=6).
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Keywords: ELISA; analysis; chicken muscle; immunoassay; roxarsone

Document Type: Research Article

Affiliations: USDA-ARS Biosciences Research Laboratory, Fargo, ND, USA

Publication date: June 1, 2011

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