A competitive indirect enzyme-linked immunoassay (ciELISA) based on a monoclonal antibody was developed to detect medroxyprogesterone acetate (MPA) in sheep intestines, which are used as sausage casings. MPA has a relative molecular mass of only 344.5, and it has no immunogenicity.
By activating MPA with o-carboxymethyl hydroxylamine hemi HCl, a hapten of MPA (3-CMO-MPA) was synthesised and linked to bovine serum albumin (BSA) and ovalbumin (OVA) to prepare artificial antigens. The monoclonal antibody was prepared from mice immunised with MPA-BSA. The ELISA method allowed
MPA detection in a range of 0.1-24.3 ng/ml with an average IC50 value of 1.7 ng/ml. The recoveries of MPA from spiked intestinal tissues at levels of 0.5-5 ng/g ranged from 85.2% to 115.1% with CVs of 8.8-11.2%, and the detection limits were 0.5 ng/g in intestinal tissues. The ciELISA test
proved to be a rapid, sensitive, economical and reliable method for screening MPA residues in intestinal tissues.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
Document Type: Research Article
Ocean University of China, Qingdao, P.R. China,Key Laboratory of Aquatic Ecology and Aquaculture of Tianjin, Tianjin Agricultural University, Tianjin, P.R. China
National Marine Environmental Monitoring Center, Dalian, P.R. China
Key Laboratory of Aquatic Ecology and Aquaculture of Tianjin, Tianjin Agricultural University, Tianjin, P.R. China
June 1, 2011
More about this publication?