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Development of an enzyme-linked immunosorbent assay for the pyrethroid fenpropathrin

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An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for the detection of fenpropathrin was developed. Two haptens, FPa (α-carboxy-3-phenoxyphenyl-2,2,3,3-tetramethyl-cyclopropane-carboxylate) and FPb (α-(N-butyrical)-3-phenoxybenzyl-2,2,3,3-tetramethyl-cyclopropane-carboxylate), were synthesised and conjugated with ovalbumin (OVA) by the mixed anhydride method as coating antigens (FPa-OVA and FPb-OVA), and the hapten FPb was conjugated to bovine serum albumin (BSA) by the carbodimide method to produce an immunogen (FPb-BSA). Polyclonal antibody against fenpropathrin was raised for screening the more sensitive coating antigen. Under optimised assay conditions, the 50% inhibitory concentration (IC50) was 0.34±0.090 mg/L and the limit of detection (LOD) was 0.0093±0.00065 mg/L. The cross-reactivities with other pyrethroids, such as deltamethrin, cypermethrin, fenvalerate and cyhalothrin, were all lower than 0.1%. Water samples spiked with different concentrations of fenpropathrin (0.01-1.0 mg/L) were analysed according to this method. The ic-ELISA developed could successfully be applied to residue analysis of fenpropathrin in aquatic.
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Keywords: ELISA; fenpropathrin; hapten; polyclonal antibody; pyrethroid

Document Type: Research Article

Affiliations: 1: Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing, P.R. China 2: Institute for the Control of Agrochemicals, Ministry of Agriculture, Beijing, P.R. China

Publication date: March 1, 2011

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