An enzyme-linked immunosorbent assay method was developed here for benzophenone (BZP) detection in package paper samples based on polyclonal antibody. 4-Aminobenzophenone (ABZP) was used as hapten and conjugated with bovine serum albumin for antibody production. Cross-reactivity results showed that the antibody can be recognised BZP, ABZP and Michler's ketone very well (IC50 value was 31.2±7.8 ng/mL, 35.9±8.3 ng/mL and 43.7±8.2 ng/mL, respectively). After optimisation of the effects from pH, ionic strength and organic solvents on immunoassay, we established a rapid method and used for BZP detection in fortified package paper samples with good recovery data (98.5 and 99.7%) at 100 and 600 ng/g spiked level.)
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package paper samples;
Document Type: Research Article
School of Food Science and Technology, Jiangnan University, Wuxi, China
School of Food Science and Technology, Jiangnan University, Wuxi, China,State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Human Entry-Exit Inspection and Quarantine Bureau, Changsha, PR China
March 1, 2011
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