A lateral flow immunoassay (LFIA) and a competitive indirect ELISA (ciELISA) were developed and validated for the quantitative analysis of sulfaquinoxaline (SQX) in chicken tissues. The limits of quantification of ciELISA were 5 ng/ml for muscle samples and 12.5 ng/ml for liver samples; recoveries in tissues spiked with SQX at 25, 40 and 50 ng/g were 73.4-87.4% (n=5). With LFIA, no matrix effect was noticed during sample analyses. The optimisation of components allowed a detection limit of 0.5 ng/ml in tissues, which was 10-25 times lower than ciELISA. The recoveries in tissues fortified at levels 5, 10, 25 and 50 ng/g were 75.8-98% (n=5). Using HPLC as confirmatory method, 70 chicken muscle and liver samples from animal experiments were analysed by two immunoassays. The assay time for LFIA was only five min, whereas the analyses by ciELISA and HPLC took several hours. Comparison of ELISA, LFIA and HPLC data showed good correlations (r2=0.99).
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
Document Type: Research Article
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, PR China
Hubei Provincial Institute of Veterinary Drug Control, Wuhan, PR China
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, PR China,Xinyang Agricultural College, Xinyang, PR China
March 1, 2011
More about this publication?