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Monoclonal antibody-based fluorescence polarisation immunoassay for nifursol in feed

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Novel tracers were designed and then used to develop a rapid, specific and sensitive fluorescence polarisation immunoassay (FPIA) method to detect nifursol in feed. The 3-((2-(2-hydroxy-3,5-dinitrobenzoyl)hydrazono)methyl)benzoic acid was used for the production of immunogen and 3,5-dinitrosalicylic acid was used for synthesis of fluorescein-labelled tracer. Based on monoclonal antibody and tracer, an optimised FPIA method was established with IC50 of 5.0 ng mL-1, and low cross-reactivity with other nitrofurans (<0.05%). FPIA provides a suitable means for screening of a large number of samples. The limits of detection calculated from the analysis of 20 known negative feed samples (pig feed, chicken feed and fish feed) were 0.4-0.8 ng g-1 (mean + 3 SD). Recoveries of nifursol fortified ranged from 85.0 to 95.0%. The coefficients of variation were less than 10%.
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Keywords: FPIA; feed detection; monoclonal antibody; nifursol; tracer design

Document Type: Research Article

Affiliations: 1: Enlife Service Co. Ltd., Shenzhen, P.R. China,Food Quality and Safety Key Laboratory, Department of Education of Guangdong Province, Institute of Food Quality and Safety, South China Agricultural University, Guangzhou, P.R. China 2: Food Quality and Safety Key Laboratory, Department of Education of Guangdong Province, Institute of Food Quality and Safety, South China Agricultural University, Guangzhou, P.R. China 3: Enlife Service Co. Ltd., Shenzhen, P.R. China

Publication date: June 1, 2010

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