Allergenic and antigenic activity of cow milk substitutes by ELISA and basophil-activation assays
We conducted enzyme-linked immunosorbent assay (ELISA) and basophil-activation assay in order to investigate the presence of allergens in selected cow milk (CM) substitutes. Our results showed the presence of residual antigenicity and allergenicity. The cross-reactivity between proteins from different animal species was observed. CM allergen-induced histamine release was dose-dependent. Therefore, differences in the relative percentage of major allergens may lead to a discrepancy between allergenic and antigenic activity. The presence of very similar epitopes could explain the cross-reactivity in mammalian milks.
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cow milk substitute
Document Type: Research Article
College of Food Science & Nutritional Engineering, China Agricultural University, Haidian, Beijing, P.R. China
College of Chemical & Environmental Engineering, Beijing Technology & Business University, Haidian, Beijing, P.R. China
Food Microbiology and Biotechnology Laboratory, North Carolina A&T State University, Greensboro, NC, USA
Department of Dermatology, Peking University Third Hospital, Haidian, Beijing, P.R. China
June 1, 2010
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