An antibody response to cholera toxin, but not soy proteins, following oral administration of adjuvanted soybean formulations
In the present study, we questioned whether an oral formulation made from soybean seeds and containing the mucosal adjuvant, cholera toxin (CT), could induce any detectable antibody responses against soy proteins. Groups of mice were repeatedly gavaged with formulations containing high (15 mg) and low (1 mg) doses of soybean seed protein extracts, along with CT as an oral adjuvant. While robust mucosal and systemic antibody responses against CT were observed, no detectable IgA, IgM, IgG or IgE anti-soy protein antibodies could be found. Furthermore, mice in all treatment groups remained healthy and continued to gain weight over the course of the study. Collectively, these data demonstrate that oral soybean-based vaccine formulations do not induce detectable antibody responses to soy proteins, even when the formulations contain a potent mucosal adjuvant. These studies underscore the safety of oral vaccine formulations which might contain soy proteins as a component.
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Document Type: Research Article
Affiliations: Department of Biology, University of North Carolina at Charlotte, Charlotte, NC, USA
Publication date: December 1, 2009