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Comparative study on the stability of soybean (Glycine max) -conglycinin in vivo

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The immunoreactivity and structural variation of -conglycinin in digesta from digestive tracts of pigs were measured by inhibition ELISA and sodium dodecyl sulphate polyacrylamide gel electrophoresis, respectively. Results showed that the immunoreactivity disappearance proportion of -conglycinin significantly increased from stomach to the caecum in all groups (P<0.05). In the stomach, upper-jejunum and middle-jejunum, the immunoreactivity disappearance proportion of -conglycinin significantly increased among these three groups (P<0.05), while it has no significant difference in ileum and caecum (P>0.05). The α', α subunits of -conglycinin were easier to digest than the  subunit. It indicated that immunoreactivity disappearance proportion of -conglycinin tended to increase with the growth of age and the descending down of digestive tract, while the  subunits of -conglycinin are more stable to digestion than α', α subunits.
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Keywords: SDS-PAGE; immunoreactivity; inhibition ELISA; pigs; soybean (Glycine max) -conglycinin; structural variation

Document Type: Research Article

Affiliations: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Jilin Agricultural University, Changchun, P.R. China

Publication date: December 1, 2009

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