Skip to main content
padlock icon - secure page this page is secure

Multi-residue detection of benzodiazepines by ELISA based on class selective antibodies

Buy Article:

$63.00 + tax (Refund Policy)

A class selective enzyme-linked immunosorbent assay (ELISA) was developed for the detection of benzodiazepines, such as nitrazepam, clonazepam, 7-aminonitrazepam, 7-amidoclonzepam, chlordiazepoxide, delorazepam and nordazepam. Class selective artificial antigens were prepared by haptens of 7-aminonitrazepam and 7-aminoclonazepam conjugating to the carrier protein bovine serum albumin (BSA). Class selective polyclonal antibodies (PcAbs) were generated by immunising male New Zealand white rabbits with the class selective immunogens. Characterisation studies of the PcAbs showed that PcAbs raised by 7-aminonitrazepam-BSA had high affinity and specificity to the six relative benzodiazepines, while PcAbs raised by 7-amidoclonazepam-BSA had high affinity and specificity to five. So, PcAbs raised by 7-aminonitrazepam-BSA were selected to establish an indirect competitive ELISA for multi-residue determination. The IC50s of the optimised immunoassay were 2.50 µg/l, 3.13 µg/l, 1.92 µg/l, 2.78 µg/l, 3.13 µg/l and 4.55 µg/l for nitrazepam, clonazepam, 7-aminonitrazepam, 7-aminoclonazepam, delorazepam and nordazepam, respectively. Assay performance was validated using spiked pig urine by adding nirazepam. Recoveries were between 75 and 103%, and the coefficient variation was below 14%. This assay can be applied to detect multi-residues of benzodiazepines in urine.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: ELISA; benzodiazepines; immunoassay; multi-residues

Document Type: Research Article

Affiliations: School of Food Science and Technology, Jiangnan University, Wuxi, China

Publication date: December 1, 2009

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more