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EspA expressed in lettuce stimulated high immunisation and had a protective effect on HeLa cells

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EspA proteins are virulence factors which are expressed by attaching and effacing Escherichia coli. In this study, BALB/C mice were immunised by a lettuce-derived EspA, and mice sera were assayed for the disruption of E. coli O157:H7 interaction with the host cell. BALB/C mice which were immunised with lettuce-derived EspA produced specific antibody titres with the highest immunoglobulin A titre of 3200 and IgG titre of 12,000. The polyclonal mice antisera blocked E. coli O157:H7-induced host cell actin re-arrangement and could label E. coli O157:H7 EspA filaments in vitro. Our present findings suggest that lettuce may be a good candidate to produce plant-derived proteins, and thus signifies the possibility of developing EspA-lettuce vaccine in defence of E. coli O157:H7 infection.
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Keywords: antibody; immunisation; mice; plants; polyclonal; sera; vaccine

Document Type: Research Article

Affiliations: 1: State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong, P.R. China 2: Department of Chemistry and Biochemistry, Ohio State University, Columbus, OH, USA 3: State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong, P.R. China,Department of Chemistry and Biochemistry, Ohio State University, Columbus, OH, USA

Publication date: September 1, 2009

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