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Detection of bovine milk adulterated with cheese whey by western blot immunoassay

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Milk processor industries and distributors have problems of milk adulteration with cheese whey. The most frequently used method to detect cheese whey is the identification of a glycomacropeptide (GMP) which is present only in cheese whey but not in milk. At present, methods to detect GMP require major work and time, presenting problems of sensitivity and accuracy at low concentrations. We developed a novel western blot analysis for GMP detection, using anti-GMP polyclonal antibodies. This assay is easy, rapid, sensitive and specific and can detect 0.001% (w/v) GMP, 0.5% (v/v) liquid cheese whey and 0.001% w/v dehydrated cheese whey. Furthermore, it can be used successfully to detect GMP in various food matrices containing cheese whey.
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Keywords: Western blot; cheese whey; glycomacropeptide; milk adulteration; polyclonal antibodies

Document Type: Research Article

Affiliations: 1: Departmento de Ingenieria Bioquimica, Centro de Ciencias Basicas, Universidad Autonoma de Aguascalientes, Mexico 2: Departamento de Microbiologia, Centro de Ciencias Basicas, Universidad Autonoma de Aguascalientes, Mexico 3: Departmento de Medicina Molecular y Bioprocesos, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Mexico

Publication date: December 1, 2008

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