A class-specific polyclonal antibody against fluoroquinolones was obtained by immunising rabbits with a novel designed hapten, which preserved the essential group of fluoroquinolones. With this specific polyclonal antibody, 13 fluoroquinolones antibiotics were simultaneously detected in indirect competitive enzyme-linked immunosorbent assay (ic-ELISA). The established ic-ELISA was class-specific to detect 13 fluoroquinolones such as norfloxacin, ciprofloxacin, ofloxacin etc., and the limit of detection (LOD) desirable was (0.01-10 ng/ml) and the acceptable recovery range was between 62 and 95%. Results proved that this group-specific ic-ELISA method was reliable and suitable for multi-residue determinations of 13 fluoroquinolones.
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Document Type: Research Article
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China,School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu, China
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
December 1, 2008
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