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Production of new class-specific polyclonal antibody for determination of fluoroquinolones antibiotics by indirect competitive ELISA

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A class-specific polyclonal antibody against fluoroquinolones was obtained by immunising rabbits with a novel designed hapten, which preserved the essential group of fluoroquinolones. With this specific polyclonal antibody, 13 fluoroquinolones antibiotics were simultaneously detected in indirect competitive enzyme-linked immunosorbent assay (ic-ELISA). The established ic-ELISA was class-specific to detect 13 fluoroquinolones such as norfloxacin, ciprofloxacin, ofloxacin etc., and the limit of detection (LOD) desirable was (0.01-10 ng/ml) and the acceptable recovery range was between 62 and 95%. Results proved that this group-specific ic-ELISA method was reliable and suitable for multi-residue determinations of 13 fluoroquinolones.
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Keywords: antibiotic; detection; fluoroquinolones; ic-ELISA; multi-residues

Document Type: Research Article

Affiliations: 1: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China,School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu, China 2: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China

Publication date: December 1, 2008

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